Translational_Unit
RBS+eGFP+H

Part:BBa_K193204:Design

Designed by: Nao Nakatani   Group: iGEM09_Tokyo_Tech   (2009-10-14)


EGFP(His tag+) downstream with RBS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 755
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 755
    Illegal NheI site found at 773
    Illegal NotI site found at 785
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 755
    Illegal BamHI site found at 551
    Illegal XhoI site found at 452
    Illegal XhoI site found at 794
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 755
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 755
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used biobrick standard assembly.



Source

We used B0030 and K193202.

References